|
Post by nomihoudai on Jan 21, 2011 12:04:45 GMT -8
|
|
|
|
Post by maurizio on Jan 21, 2011 22:39:14 GMT -8
At best of my knowledge, only Canon SRL digital cameras have live view facility, so your choice is restricted to this brand. I add that I doubt you would ever obtain a resolution up to wing scales of a Tortricidae with a 50 mm macro lens.
|
|
|
Post by Chris Grinter on Jan 21, 2011 23:03:07 GMT -8
|
|
|
Post by nomihoudai on Jan 22, 2011 2:06:22 GMT -8
Hi Chris, of course I have seen your website, it is so well known now that it pops up on second place when you search "microlepidoptera" on google. com ! The Canon 40D looks already nice, and would be in the price range I set for the camera itself, 400 euro to max. 600 euro. Ok now to your site Chris, I saw some example pics of Gnorimoschema sedgwickensis, the pic does look great indeed but it is for me of rather large size ( 15 mm wingspan ) and it has no metallic colors, I think metallic scales are another thing that gives you a pain in the ass when taking a pic and get propper lightning down to the levels I want it to get. I will attach to this post a pic of a Tortricidae I made with the cam of a friend ( Nikon D700, a common 500mm, and 3 rings to change the focal length, I think I got it down to somewhat 60 or 70 mm). You see that it is resolution wise pretty close to yours, you can see the scales ( the lightning is s*h*i*t*ty but that is another topic ). I also saw your post on how to light the specimen, I will keep that method in consideration and try to do it myself, but I would also like to hear if people have different methods, solutions. ...well as I got now people into the discussion I also want to go a little bit in the math part of the topic, I am about to do my diploma in physics,... nevertheless I suck at optics and need people to confirm if I got it right or not ;D I will upload now a few pics in differrent posts, I coudl upload them into 1 post using flickr but then they may get lost when I delete them on flickr and I hope the topic will grow large, good and helpful and should thus be conserved for later. Here the 18 mm Tortricidae: Attachments:
|
|
|
Post by nomihoudai on Jan 22, 2011 2:18:11 GMT -8
Ok and here comes problem case number 1, an unidientified 3 mm long winged micro, now with the Nikon I can see the markings of the wings a little bit but yet... I can't see the border and the tip of it properly, furthermore it looks horrible and can't be showed anywhere ( overlook the fact that the left side is in a mess ... )
|
|
|
Post by nomihoudai on Jan 22, 2011 2:22:10 GMT -8
and problem case number 2, an Yponomeutidae, Argyreshtiinae, Argyresthia goedartella. It has metallic scales and it makes the pic even more blurry than before. Also pic taken with the Nikon D700
|
|
|
|
Post by nomihoudai on Jan 22, 2011 2:23:52 GMT -8
... and now for the math part, with the previously shown lens of 300 Euro with 50 mm macro I come to a smallest spot resolution of 2 micrometer, can anyone confirm that, and can anyone tell me some numbers for regular butterfly wing scales and for micromoth wing scales ? Here in large:
|
|
|
Post by maurizio on Jan 22, 2011 9:18:25 GMT -8
I think you have to do stack. Here I am posting an image at low resolution of a Buprestidae (Acmaeoderella gibbosula). It is 3 mm in leght. I obtained the image with a Nikon D90, 3 Kenko extension tubes, 85 mm MicroNikkor lens and ring flash Nikon SB-29. BUT I had to take about 10 shots in sequence at different focus depth, and later I obtained an all-in-focus image with CombineZM, a freeware software of stacking. Light is also important, as correctly underlined by Chris in his interesting posting. I use as diffuser a plastic glass without the bottom. It's enough to obtain a diffuse light, less shadows and a good resolution of scales. Uploaded with ImageShack.us
|
|
|
Post by Chris Grinter on Jan 22, 2011 17:59:27 GMT -8
You're on the right track Claude - I think lighting helps a whole lot. At home I don't use a flash but that would be ideal. With a low f-stop, the shutter speed lags so everything has to remain very still. I've been taking images in my lab at the museum lately and gotten some spectacular images of even small moths. Attached is a nepticulidae - 3mm - think it came out pretty nice. That is from a single image, no stacking. Canon 50d - and some crazy tube attachments - and twin studio flash with a styrofoam cup as a diffuser. Without the flash and tube extensions I don't think I could get this image at home. Photographing shiny or metallic specimens is always difficult, and you'll almost never capture the real luster of the specimen. You end up with something that is a bit washed out even if the lighting is great and everything is in focus. Check out the other micro - same setup as above. In life it's a blinding gold color... For live view you need a Canon - and then buy the best fixed length lens you can afford. Then mess around with lighting and f/stop until you go nuts. Then, you'll finally break through! haha
|
|
|
Post by nomihoudai on Jan 23, 2011 4:38:16 GMT -8
@ Maurizio : Yes I know about stacking and wanted to use it to, but with much less pics, the butterflies are much flatter than beetles, for beetles stackign is really important, in butterflies you just need 2-3 pics depending how thick the body is. Well I need to come up with a good solution for my light then, what I used on my pics was a box with 30 LED lights in it and I did not put anything before them to diffuse the light. @ Chris, now you got what I want to photograph, those bloody Nepticulidae. Your pic is already really good and it is nice to see the scaling on the wings. I showed my friend your post ( that studies design and is semi-professional photographer, he took the charaxes pic : insectnet.proboards.com/index.cgi?board=favorites&action=display&thread=129 ) and he said something like " ahh he used macrorings ", so apparenly there is more kinds of rings and I used the "bad" ones on my pics above. I just hope those won't become too expensive ... For the life view on laptop, as far as he told me things recently have changed and there is more camera models that feature it. The other micro does not look too good but I know how beautiful shiny they are in reality, maybe one would need an aditional light that will light from above to get the metallic reflections, maybe one day I can come up with a good method on these. There is little litterature, websites etc. with good microlepidoptera pics and Ihope I can change this a little bit in the future.
|
|
|
Post by Chris Grinter on Jan 23, 2011 4:56:45 GMT -8
The marcoring setup I've been using is over two feet long - and the only system our entire museum has. However, it's still a SLR based tech. I'm happy with the nepticulid - but the gold Dyseriocrania isn't a great shot. My next experiment will be using a Nikon microscope camera attached to a stereo microscope and do montage shots. The lighting is better once you cut out the ridiculous macro rings... and I can control it more subtly. That might be the only way to get really high quality shots I fear... have a local museum?
|
|
|
Post by nomihoudai on Jan 23, 2011 5:16:48 GMT -8
Yes Chris I do have a local museum, the Museum of Karlsruhe where I do voluntary work, with the largest Microlepidoptera collection in whole Germany ( Amsel worked there, probably you are familiar with this work as he also corresponded with the USA in his life time ) and the Museum in Luxembourg where I work as student during holidays. If I don't get a nice setup on my own I will have to ask them what they use... and there is still another method. We have a synchrotron here in Karlsruhe, I don't know if you know what that is, for those that do not know, it is a circular particle accelerator that accelerates electrons very very fast on circular orbits and they emit x-rays and synchrotron rays. The local Museum here in Karlsruhe uses the synchrotron rays to get 3D models of very very tiny beetles, I think about 1 mm, on the pic you can then see every single hair on those ... ;D ;D ;D That thing will supply nice pics: ankaweb.fzk.de/website.php?page=instrumentation_main
|
|
|
Post by jshuey on Jan 29, 2011 8:40:21 GMT -8
|
|
|
Post by nomihoudai on Jun 16, 2011 14:14:16 GMT -8
Time to digg this topic out again =D I had been busy by saving up some money and spending it again, now I can present first results of what I had planned. I went for the NikonD5000 and I choose macro bellows and a copystand as equipment, it gives great results. Tomorrow I will post more about the setup and pictures of it if you want to go for the same stuff, for now only a little teaser of what it is able to do A micro resized pic: A direct crop from a pic of Nymphalis polychloros:
|
|
|
Post by Chris Grinter on Jun 16, 2011 15:08:19 GMT -8
That's great resolution! Why the blue background though? I tend to go with the standard universal grey or sometimes a black background if the moth has white fringe.
|
|